Botryococcus braunii cells: ultrasound-intensified outdoor cultivation integrated with in situ magnetic separation

Shi-Kai Wang; Feng Wang; Amanda R Stiles; Chen Guo; Chun-Zhao Liu

doi:10.1016/j.biortech.2014.06.028

Botryococcus braunii cells: ultrasound-intensified outdoor cultivation integrated with in situ magnetic separation

Bioresour Technol. 2014 Sep:167:376-82. doi: 10.1016/j.biortech.2014.06.028. Epub 2014 Jun 16.

Authors

Shi-Kai Wang¹, Feng Wang², Amanda R Stiles³, Chen Guo⁴, Chun-Zhao Liu⁵

Affiliations

¹ National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
² National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
³ Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
⁴ National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
⁵ National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: czliu@ipe.ac.cn.

PMID: 24998478
DOI: 10.1016/j.biortech.2014.06.028

Abstract

An integrated system combining ultrasound-intensified outdoor cultivation of Botryococcus braunii with in situ magnetic harvesting of the algal cells was developed. The algal cells were cultivated in 200 L plastic bag reactors, and seven five-minute ultrasonic treatments at a four-day interval using a fixed frequency of 40 kHz and a total power of 300 W improved algal cell biomass and hydrocarbon productivity. The algal cells were harvested using functional magnetic particles and a magnetic separator, and a recovery efficiency of 90% was obtained under continuous operation at a flow rate of 100mL/min using the in situ magnetic separation system. The overall production cost using the integrated system was US$ 25.14 per kilogram of B. braunii dry biomass. The system developed in this study provides a base for the industrial production of B. braunii.

Keywords: Botryococcus braunii; Hydrocarbon; Magnetic separation; Outdoor cultivation; Ultrasound stimulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / economics
Cell Culture Techniques / methods*
Chlorophyta / growth & development*
Chlorophyta / metabolism
Hydrocarbons / metabolism
Kinetics
Lipids / biosynthesis
Magnetics / economics
Magnetics / methods*
Thermodynamics
Ultrasonics / methods*

Substances

Hydrocarbons
Lipids