Aflatoxicosis chemoprevention by probiotic Lactobacillius and lack of effect on the major histocompatibility complex

Sumit Rawal; Miranda M Bauer; Kristelle M Mendoza; Hani El-Nezami; Jeffery R Hall; Ji Eun Kim; John R Stevens; Kent M Reed; Roger A Coulombe Jr

doi:10.1016/j.rvsc.2014.06.008

Aflatoxicosis chemoprevention by probiotic Lactobacillius and lack of effect on the major histocompatibility complex

Res Vet Sci. 2014 Oct;97(2):274-81. doi: 10.1016/j.rvsc.2014.06.008. Epub 2014 Jun 13.

Authors

Sumit Rawal¹, Miranda M Bauer², Kristelle M Mendoza², Hani El-Nezami³, Jeffery R Hall¹, Ji Eun Kim¹, John R Stevens⁴, Kent M Reed², Roger A Coulombe Jr⁵

Affiliations

¹ Graduate Toxicology Program, and Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USA.
² Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
³ School of Biological Sciences, University of Hong Kong, Hong Kong, China.
⁴ Department of Mathematics and Statistics, Utah State University, Logan, UT 84322, USA.
⁵ Graduate Toxicology Program, and Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, UT 84322, USA. Electronic address: roger@usu.edu.

PMID: 24997556
DOI: 10.1016/j.rvsc.2014.06.008

Abstract

Turkeys are extremely sensitive to aflatoxin B1 (AFB1) which causes decreased growth, immunosuppression and liver necrosis. The purpose of this study was to determine whether probiotic Lactobacillus, shown to be protective in animal and clinical studies, would likewise confer protection in turkeys, which were treated for 11 days with either AFB1 (AFB; 1 ppm in diet), probiotic (PB; 1 × 10(11) CFU/ml; oral, daily), probiotic + AFB1 (PBAFB), or PBS control (CNTL). The AFB1 induced drop in body and liver weights were restored to normal in CNTL and PBAFB groups. Hepatotoxicity markers were not significantly reduced by probiotic treatment. Major histocompatibility complex (MHC) genes BG1 and BG4, which are differentially expressed in liver and spleens, were not significantly affected by treatments. These data indicate modest protection, but the relatively high dietary AFB1 treatment, and the extreme sensitivity of this species may reveal limits of probiotic-based protection strategies.

Keywords: Aflatoxin; BG; Chemoprevention; Gene expression; MHC; Poultry; Probiotics; Toxicology; Turkeys.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Aflatoxin B1 / administration & dosage
Aflatoxin B1 / toxicity*
Animals
Chemoprevention / methods
Chemoprevention / veterinary
Diet / adverse effects
Foodborne Diseases / pathology
Foodborne Diseases / prevention & control
Foodborne Diseases / veterinary*
Lactobacillus*
Liver / drug effects
Liver / pathology
Major Histocompatibility Complex / drug effects*
Major Histocompatibility Complex / genetics
Mycotoxicosis / pathology
Mycotoxicosis / prevention & control
Mycotoxicosis / veterinary*
Organ Size / drug effects
Poultry Diseases / immunology
Poultry Diseases / pathology
Poultry Diseases / prevention & control*
Probiotics / pharmacology*
Probiotics / therapeutic use*
Species Specificity
Spleen / drug effects
Spleen / pathology
Treatment Outcome
Turkeys

Substances

Aflatoxin B1