Tissue-specific proteome tagging: an orthogonal approach

Adrienne L Snyder; Eric M Brustad

doi:10.1002/cbic.201402210

Tissue-specific proteome tagging: an orthogonal approach

Chembiochem. 2014 Aug 18;15(12):1731-3. doi: 10.1002/cbic.201402210. Epub 2014 Jul 2.

Authors

Adrienne L Snyder¹, Eric M Brustad

Affiliation

¹ Department of Chemistry, Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, 125 South Road, Chapel Hill, NC 27599 (USA).

PMID: 24990619
DOI: 10.1002/cbic.201402210

Abstract

SORTing through the proteome: A versatile method has been developed for the incorporation of a novel 1,3-disubstituted cyclopropene amino acid into the proteome in response to a variety of sense codons and subsequent labeling. Incorporation can be confined to certain tissues by the selective expression of an orthogonal tRNA synthetase under cell-selective promoters.

Keywords: bio-orthogonal chemistry; cycloaddition; genetic code expansion; proteomics; synthetases.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Amino Acids / chemistry
Amino Acids / genetics
Amino Acids / metabolism*
Amino Acyl-tRNA Synthetases / metabolism
Codon / genetics
Humans
Organ Specificity / genetics
Proteome / chemistry
Proteome / genetics*
Proteome / metabolism*
Proteomics*

Substances

Amino Acids
Codon
Proteome
Amino Acyl-tRNA Synthetases