Phosphoproteomic analysis seeks to determine the overall level of protein phosphorylation, as a result of kinase and phosphatase activity, and determine the identity of proteins which are phosphorylated and the amino acid residues which hold the phosphate group. The methodologies available have improved with increased research efforts; however, the most commonly followed procedure is to enrich for phosphoproteins or peptides and undertake tandem mass spectrometric analysis focusing on specific signature losses which represent phosphopeptides. There have been many advances in this area and these are detailed both in relation to available protocols for phosphoproteomic analysis and to the widening range of biomedical fields in which such approaches are being commonly applied.
Keywords: Cancer; Immunity; Phosphopeptides; Phosphoproteomics; Stem cells.
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