Decreased miR-204 in H. pylori-associated gastric cancer promotes cancer cell proliferation and invasion by targeting SOX4

Xiaoying Zhou; Lin Li; Jing Su; Guoxin Zhang

doi:10.1371/journal.pone.0101457

Decreased miR-204 in H. pylori-associated gastric cancer promotes cancer cell proliferation and invasion by targeting SOX4

PLoS One. 2014 Jul 1;9(7):e101457. doi: 10.1371/journal.pone.0101457. eCollection 2014.

Authors

Xiaoying Zhou¹, Lin Li¹, Jing Su¹, Guoxin Zhang¹

Affiliation

¹ Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing, China; First Clinical Medical College of Nanjing Medical University, Nanjing, China.

Abstract

Background: The molecular mechanism between Helicobacter pylori (H. pylori) infection and gastric cancer remained largely unknown. In this study, we determined the role of miRNA in H. pylori induced gastric cancer.

Methods and results: We found that miR-204 was decreased in H. pylori positive tissues by qRT-PCR. Knockdown of miR-204 enhanced the invasion and proliferation ability of gastric cancer cells in vitro. Luciferase assay revealed that SOX4 was target gene of miR-204, which was found up-regulated in H. pylori positive tissues. Down-regulation of miR-204 and over-expression of SOX4 promoted epithelial-mesenchymal transition process.

Conclusion: Taken together, our findings demonstrated that miR-204 may act as a tumor suppressor in H. pylori induced gastric cancer by targeting SOX4.

Publication types

Clinical Trial
Research Support, Non-U.S. Gov't
Retracted Publication

MeSH terms

Adult
Aged
Cell Proliferation / genetics*
Epithelial-Mesenchymal Transition
Female
Genes, Tumor Suppressor*
Helicobacter Infections* / genetics
Helicobacter Infections* / metabolism
Helicobacter Infections* / pathology
Helicobacter pylori*
Humans
Male
MicroRNAs* / biosynthesis
MicroRNAs* / genetics
Middle Aged
Neoplasm Invasiveness
Neoplasm Proteins* / genetics
Neoplasm Proteins* / metabolism
RNA, Neoplasm* / biosynthesis
RNA, Neoplasm* / genetics
SOXC Transcription Factors* / genetics
SOXC Transcription Factors* / metabolism
Stomach Neoplasms* / genetics
Stomach Neoplasms* / metabolism
Stomach Neoplasms* / microbiology
Stomach Neoplasms* / pathology

Substances

MIRN204 microRNA, human
MicroRNAs
Neoplasm Proteins
RNA, Neoplasm
SOX4 protein, human
SOXC Transcription Factors

Grants and funding

Guoxin Zhang was funded by National Natural Science Foundation of China (No. 81270476) (http://www.nsfc.gov.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.