Genome sequence of Ensifer medicae strain WSM1369; an effective microsymbiont of the annual legume Medicago sphaerocarpos

Jason Terpolilli; Giovanni Garau; Yvette Hill; Rui Tian; John Howieson; Lambert Bräu; Lynne Goodwin; James Han; Konstantinos Liolios; Marcel Huntemann; Amrita Pati; Tanja Woyke; Konstantinos Mavromatis; Victor Markowitz; Natalia Ivanova; Nikos Kyrpides; Wayne Reeve

doi:10.4056/sigs.4838624

Genome sequence of Ensifer medicae strain WSM1369; an effective microsymbiont of the annual legume Medicago sphaerocarpos

Stand Genomic Sci. 2013 Dec 17;9(2):420-30. doi: 10.4056/sigs.4838624. eCollection 2013 Dec 20.

Authors

Jason Terpolilli¹, Giovanni Garau², Yvette Hill¹, Rui Tian¹, John Howieson¹, Lambert Bräu³, Lynne Goodwin⁴, James Han⁵, Konstantinos Liolios⁵, Marcel Huntemann⁵, Amrita Pati⁵, Tanja Woyke⁵, Konstantinos Mavromatis⁶, Victor Markowitz⁶, Natalia Ivanova⁵, Nikos Kyrpides⁵, Wayne Reeve¹

Affiliations

¹ Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
² Dipartimento di Agraria, S.T.A.A., University of Sassari, Italy.
³ School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
⁴ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
⁵ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁶ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Abstract

Ensifer medicae WSM1369 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago. WSM1369 was isolated in 1993 from a nodule recovered from the roots of Medicago sphaerocarpos growing at San Pietro di Rudas, near Aggius in Sardinia (Italy). WSM1369 is an effective microsymbiont of the annual forage legumes M. polymorpha and M. sphaerocarpos. Here we describe the features of E. medicae WSM1369, together with genome sequence information and its annotation. The 6,402,557 bp standard draft genome is arranged into 307 scaffolds of 307 contigs containing 6,656 protein-coding genes and 79 RNA-only encoding genes. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.