Genome sequence of the Lebeckia ambigua-nodulating "Burkholderia sprentiae" strain WSM5005(T.)

Wayne Reeve; Sofie De Meyer; Jason Terpolilli; Vanessa Melino; Julie Ardley; Tian Rui; Ravi Tiwari; John Howieson; Ron Yates; Graham O'Hara; Megan Lu; David Bruce; Chris Detter; Roxanne Tapia; Cliff Han; Chia-Lin Wei; Marcel Huntemann; James Han; I-Min Chen; Konstantinos Mavromatis; Victor Markowitz; Ernest Szeto; Natalia Ivanova; Natalia Mikhailova; Galina Ovchinnikova; Ioanna Pagani; Amrita Pati; Lynne Goodwin; Lin Peters; Sam Pitluck; Tanja Woyke; Nikos Kyrpides

doi:10.4056/sigs.4558268

Genome sequence of the Lebeckia ambigua-nodulating "Burkholderia sprentiae" strain WSM5005(T.)

Stand Genomic Sci. 2013 Dec 15;9(2):385-94. doi: 10.4056/sigs.4558268. eCollection 2013 Dec 20.

Authors

Wayne Reeve¹, Sofie De Meyer¹, Jason Terpolilli¹, Vanessa Melino¹, Julie Ardley¹, Tian Rui¹, Ravi Tiwari¹, John Howieson¹, Ron Yates², Graham O'Hara¹, Megan Lu³, David Bruce³, Chris Detter³, Roxanne Tapia³, Cliff Han³, Chia-Lin Wei³, Marcel Huntemann³, James Han³, I-Min Chen⁴, Konstantinos Mavromatis³, Victor Markowitz⁴, Ernest Szeto⁴, Natalia Ivanova³, Natalia Mikhailova³, Galina Ovchinnikova³, Ioanna Pagani³, Amrita Pati³, Lynne Goodwin⁴, Lin Peters³, Sam Pitluck³, Tanja Woyke³, Nikos Kyrpides³

Affiliations

¹ Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
² Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia ; Department of Agriculture and Food, Western Australia, Australia.
³ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁴ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.

Abstract

"Burkholderia sprentiae" strain WSM5005(T) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated in Australia from an effective N2-fixing root nodule of Lebeckia ambigua collected in Klawer, Western Cape of South Africa, in October 2007. Here we describe the features of "Burkholderia sprentiae" strain WSM5005(T), together with the genome sequence and its annotation. The 7,761,063 bp high-quality-draft genome is arranged in 8 scaffolds of 236 contigs, contains 7,147 protein-coding genes and 76 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.

Keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.