Genome sequence of the South American clover-nodulating Rhizobium leguminosarum bv. trifolii strain WSM597

Wayne Reeve; Jason Terpolilli; Vanessa Melino; Julie Ardley; Rui Tian; Sofie De Meyer; Ravi Tiwari; Ronald Yates; Graham O'Hara; John Howieson; Mohamed Ninawi; Brittany Held; David Bruce; Chris Detter; Roxanne Tapia; Cliff Han; Chia-Lin Wei; Marcel Huntemann; James Han; I-Min Chen; Konstantinos Mavromatis; Victor Markowitz; Natalia Ivanova; Galina Ovchinnikova; Ioanna Pagani; Amrita Pati; Lynne Goodwin; Tanja Woyke; Nikos Kyrpides

doi:10.4056/sigs.4508258

Genome sequence of the South American clover-nodulating Rhizobium leguminosarum bv. trifolii strain WSM597

Stand Genomic Sci. 2013 Dec 15;9(2):264-72. doi: 10.4056/sigs.4508258. eCollection 2013 Dec 20.

Authors

Wayne Reeve¹, Jason Terpolilli¹, Vanessa Melino¹, Julie Ardley¹, Rui Tian¹, Sofie De Meyer¹, Ravi Tiwari¹, Ronald Yates², Graham O'Hara¹, John Howieson¹, Mohamed Ninawi¹, Brittany Held³, David Bruce³, Chris Detter³, Roxanne Tapia³, Cliff Han³, Chia-Lin Wei³, Marcel Huntemann³, James Han³, I-Min Chen⁴, Konstantinos Mavromatis³, Victor Markowitz⁴, Natalia Ivanova³, Galina Ovchinnikova³, Ioanna Pagani³, Amrita Pati³, Lynne Goodwin⁵, Tanja Woyke³, Nikos Kyrpides³

Affiliations

¹ Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
² Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia ; Department of Agriculture and Food, Western Australia, Australia.
³ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁴ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
⁵ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.

Abstract

Rhizobium leguminosarum bv. trifolii strain WSM597 is an aerobic, motile, Gram-negative, non-spore-forming rod isolated from a root nodule of the annual clover Trifolium pallidum L. growing at Glencoe Research Station near Tacuarembó, Uruguay. This strain is generally ineffective for nitrogen (N2) fixation with clovers of Mediterranean, North American and African origin, but is effective on the South American perennial clover T. polymorphum Poir. Here we describe the features of R. leguminosarum bv. trifolii strain WSM597, together with genome sequence information and annotation. The 7,634,384 bp high-quality-draft genome is arranged in 2 scaffolds of 53 contigs, contains 7,394 protein-coding genes and 87 RNA-only encoding genes, and is one of 20 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Community Sequencing Program.

Keywords: Alphaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.