Calcium ion supplementation increases brook trout Salvelinus fontinalis spermatozoa activation at the end of the spawning season

O Bondarenko; B Dzyuba; J Cosson; M Rodina; O Linhart

doi:10.1111/jfb.12445

Calcium ion supplementation increases brook trout Salvelinus fontinalis spermatozoa activation at the end of the spawning season

J Fish Biol. 2014 Sep;85(3):933-7. doi: 10.1111/jfb.12445. Epub 2014 Jun 27.

Authors

O Bondarenko¹, B Dzyuba, J Cosson, M Rodina, O Linhart

Affiliation

¹ Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic.

PMID: 24976556
DOI: 10.1111/jfb.12445

Abstract

The role of environmental ion composition and osmolality in calcium ion (Ca(2+) ) signalling of spermatozoa activation over the course of the spawning period of brook trout Salvelinus fontinalis was investigated. Motility at the end of spawning was low (mean ± s.d. of 5 ± 2% motile spermatozoa with curvilinear velocity of 25 ± 8 µm s(-1) ). Addition of 10 mM Ca(2+) to the activation medium resulted in values similar to those recorded during the middle of the spawning period (mean ± s.d. of 95 ± 6% motile spermatozoa with curvilinear velocity of 130 ± 15 µm s(-1) ).

Keywords: salmonid; semen quality; sperm motility.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium / metabolism*
Dietary Supplements
Male
Seasons
Sperm Motility / physiology*
Spermatozoa / physiology*
Trout / physiology*

Substances

Calcium