Concepts in quantitative fluorescence microscopy

Jennifer C Waters; Torsten Wittmann

doi:10.1016/B978-0-12-420138-5.00001-X

Concepts in quantitative fluorescence microscopy

Methods Cell Biol. 2014:123:1-18. doi: 10.1016/B978-0-12-420138-5.00001-X.

Authors

Jennifer C Waters¹, Torsten Wittmann²

Affiliations

¹ Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
² Department of Cell and Tissue Biology, University of California, San Francisco, USA.

PMID: 24974019
DOI: 10.1016/B978-0-12-420138-5.00001-X

Abstract

In recent years, there has been an enormous increase in the publication of spatial and temporal measurements made on fluorescence microscopy digital images. Quantitative fluorescence microscopy is a powerful and important tool in biological research but is also an error-prone technique that requires careful attention to detail. In this chapter, we focus on general concepts that are critical to performing accurate and precise quantitative fluorescence microscopy measurements.

Keywords: Fluorescence; Intensity; Measure; Microscopy; Quantify; Quantitate; Quantitation; Resolution; Sampling.

MeSH terms

Algorithms
Cells, Cultured
Image Processing, Computer-Assisted / methods*
Image Processing, Computer-Assisted / standards
Microscopy, Fluorescence / methods
Microscopy, Fluorescence / standards
Signal-To-Noise Ratio
Single-Cell Analysis / methods
Single-Cell Analysis / standards