Simultaneous determination of propofol and remifentanil in rat plasma by liquid chromatography-tandem mass spectrometry: application to preclinical pharmacokinetic drug-drug interaction analysis

Biomed Chromatogr. 2015 Mar;29(3):325-7. doi: 10.1002/bmc.3281. Epub 2014 Jun 24.

Abstract

Propofol (Pro) is an ultra-short-acting hypnotic agent used for general anesthesia that has no analgesic properties. Remifentanil (Rem) is an ultra-short-acting opioid administered concomitantly as an analgesic with Pro. To evaluate the pharmacokinetic interactions between Pro and Rem, we developed and validated a method combining high-performance liquid chromatography with tandem mass spectrometry for simultaneous determination of Pro and Rem. The proposed method was successfully used to study the pharmacokinetic interactions of Pro and Rem coadministered to rats.

Keywords: LC-MS/MS; pharmacokinetic interactions; propofol; rat plasma; remifentanil.

MeSH terms

  • Analgesics, Opioid / blood
  • Analgesics, Opioid / pharmacokinetics
  • Anesthetics, Intravenous / blood
  • Anesthetics, Intravenous / pharmacokinetics
  • Animals
  • Area Under Curve
  • Chromatography, Liquid / methods
  • Drug Interactions*
  • Hypnotics and Sedatives / blood
  • Hypnotics and Sedatives / pharmacokinetics
  • Limit of Detection
  • Male
  • Piperidines / blood*
  • Piperidines / pharmacokinetics
  • Propofol / blood*
  • Propofol / pharmacokinetics
  • Rats, Wistar
  • Remifentanil
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*

Substances

  • Analgesics, Opioid
  • Anesthetics, Intravenous
  • Hypnotics and Sedatives
  • Piperidines
  • Remifentanil
  • Propofol