Abstract
Interfacing synthetic materials with biomacromolecules provides new systems for biological applications. We report the creation of a reversible multivalent supramolecular "zipper" recognition motif between gold nanoparticles and proteins. In this assembly, carboxylate-functionalized nanoparticles interact strongly with oligohistidine tags. This interaction can be tuned through His-tag length, and offers unique binding profiles based on the pH and electrolyte concentration of the medium.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Biocompatible Materials / chemistry*
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Carboxylic Acids / chemistry*
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Cloning, Molecular
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Escherichia coli / metabolism
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Genetic Engineering
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Gold / chemistry
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Green Fluorescent Proteins / chemistry
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Histidine / chemistry*
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Hydrogen-Ion Concentration
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Ions
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Ligands
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Macromolecular Substances / chemistry
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Metal Nanoparticles / chemistry
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Nanoparticles / chemistry*
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Protein Binding
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Protein Engineering
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Proteins / chemistry*
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Recombinant Proteins / chemistry
Substances
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Biocompatible Materials
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Carboxylic Acids
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Ions
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Ligands
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Macromolecular Substances
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Proteins
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Recombinant Proteins
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Green Fluorescent Proteins
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Histidine
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Gold