Clipping of predetermined fragments from the human genome by S1 nuclease-PNA combinations

Chem Commun (Camb). 2014 Aug 14;50(63):8674-6. doi: 10.1039/c4cc01420k. Epub 2014 Jun 24.

Abstract

By combining S1 nuclease with two strands of pseudo-complementary peptide nucleic acid (pcPNA), the whole human genome was selectively cut at targeted sites, and desired fragments were clipped from the genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromosomes / chemistry
  • DNA / chemistry
  • DNA, Single-Stranded / chemistry
  • Fungal Proteins / chemistry*
  • Genome, Human*
  • Humans
  • Molecular Sequence Data
  • Peptide Nucleic Acids / chemistry*
  • Single-Strand Specific DNA and RNA Endonucleases / chemistry*
  • Telomere / chemistry

Substances

  • DNA, Single-Stranded
  • Fungal Proteins
  • Peptide Nucleic Acids
  • DNA
  • Endonuclease S1, Aspergillus
  • Single-Strand Specific DNA and RNA Endonucleases