The bond strength of ActiV GP and gutta-percha/AH Plus after using 2% CHX as final rinse was evaluated in this study. According to the final irrigation regimen forty roots were divided into two groups (n = 20) randomly: Group 1-5 ml of 17% EDTA; Group 2-5 ml of 17% EDTA followed by 5 ml of 2% chlorhexidine gluconate (CHX). Based on the obturation system the two groups were further subdivided into two subgroups (n = 10). The roots were horizontally sectioned from the coronal and midthird sections for measuring the push-out bond strength. CHX/AH Plus (2.795 ± 0.42 MPa) and EDTA/ActiV GP (1.489 ± 0.117 MPa) recorded the highest and lowest mean bond strength values respectively. It was concluded that when 2% CHX was used as a final irrigation rinse the bond strength of both gutta-percha/AH Plus and ActiV GP obturation system improved significantly.