An amorphous silicon photodiode microfluidic chip to detect nanomolar quantities of HIV-1 virion infectivity factor

Cláudia R Vistas; Sandra S Soares; Rogério M M Rodrigues; Virginia Chu; João P Conde; Guilherme N M Ferreira

doi:10.1039/c4an00695j

An amorphous silicon photodiode microfluidic chip to detect nanomolar quantities of HIV-1 virion infectivity factor

Analyst. 2014 Aug 7;139(15):3709-13. doi: 10.1039/c4an00695j.

Authors

Cláudia R Vistas¹, Sandra S Soares, Rogério M M Rodrigues, Virginia Chu, João P Conde, Guilherme N M Ferreira

Affiliation

¹ IBB-Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine (CBME), University of Algarve, Campus de Gambelas, 8000-139 Faro, Portugal. gferrei@ualg.pt.

PMID: 24922601
DOI: 10.1039/c4an00695j

Abstract

A hydrogenated amorphous silicon (a-Si:H) photosensor was explored for the quantitative detection of a HIV-1 virion infectivity factor (Vif) at a detection limit in the single nanomolar range. The a-Si:H photosensor was coupled with a microfluidic channel that was functionalized with a recombinant single chain variable fragment antibody. The biosensor selectively recognizes HIV-1 Vif from human cell extracts.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

HEK293 Cells
HIV Infections / diagnosis*
HIV Infections / virology*
HIV-1 / isolation & purification*
Humans
Microfluidic Analytical Techniques / instrumentation*
Optics and Photonics / instrumentation
Silicon / chemistry*
vif Gene Products, Human Immunodeficiency Virus / isolation & purification*

Substances

vif Gene Products, Human Immunodeficiency Virus
Silicon