Fabry-Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis

Appl Opt. 2014 May 10;53(14):2983-7. doi: 10.1364/AO.53.002983.

Abstract

We demonstrate the ability of our hyperspectral imaging device, based on a scanning Fabry-Perot interferometer, to obtain a single hyper-image of a sample marked with different fluorescent molecules, and to unambiguously discriminate them by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein, and quantum dots emitting in the yellow-orange region, demonstrates the feasibility of multi-labeled fluorescence microscopy without the use of multiple filter sets or dispersive means.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry*
  • Fourier Analysis
  • Image Enhancement / instrumentation*
  • Interferometry / instrumentation*
  • Microscopy, Fluorescence, Multiphoton / instrumentation*
  • Molecular Imaging / instrumentation*

Substances

  • Fluorescent Dyes