An optimized HPLC method for soil fungal biomass determination and its application to a detritus manipulation study

Aron Beni; Erzsebet Soki; Kate Lajtha; Istvan Fekete

doi:10.1016/j.mimet.2014.05.022

An optimized HPLC method for soil fungal biomass determination and its application to a detritus manipulation study

J Microbiol Methods. 2014 Aug:103:124-30. doi: 10.1016/j.mimet.2014.05.022. Epub 2014 Jun 8.

Authors

Aron Beni¹, Erzsebet Soki², Kate Lajtha³, Istvan Fekete²

Affiliations

¹ Institute of Environmental Sciences, College of Nyiregyhaza, Sóstói u 31/B, Nyiregyhaza, Hungary. Electronic address: beniaron@nyf.hu.
² Institute of Environmental Sciences, College of Nyiregyhaza, Sóstói u 31/B, Nyiregyhaza, Hungary.
³ Oregon State University, Department of Crop and Soil Science, 3017 Agricultural and Life Sciences Building, Corvallis, OR 97331, USA.

PMID: 24918988
DOI: 10.1016/j.mimet.2014.05.022

Abstract

The goal of this research was to develop modified analytical method for the quantitative analysis of ergosterol, which is highly effective marker for fungal biomass. We suggest that our optimized method for the determination of ergosterol is an effective way to monitor changes in fungal biomass under different environmental conditions.

Keywords: DIRT; Ergosterol; Fungal biomass; HPLC; Litter manipulation; SPE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomass*
Chromatography, High Pressure Liquid*
Environment
Ergosterol / analysis
Ergosterol / chemistry
Forests
Fungi / chemistry*
Reproducibility of Results
Soil Microbiology*

Substances

Ergosterol