Dissemination in Japan of multidrug-resistant Pseudomonas aeruginosa isolates producing IMP-type metallo-β-lactamases and AAC(6')-Iae/AAC(6')-Ib

Masayoshi Tojo; Tatsuya Tada; Masahiro Shimojima; Masashi Tanaka; Kenji Narahara; Tohru Miyoshi-Akiyama; Teruo Kirikae; Norio Ohmagari

doi:10.1016/j.jiac.2014.04.014

Dissemination in Japan of multidrug-resistant Pseudomonas aeruginosa isolates producing IMP-type metallo-β-lactamases and AAC(6')-Iae/AAC(6')-Ib

J Infect Chemother. 2014 Sep;20(9):586-8. doi: 10.1016/j.jiac.2014.04.014. Epub 2014 Jun 6.

Authors

Masayoshi Tojo¹, Tatsuya Tada², Masahiro Shimojima³, Masashi Tanaka⁴, Kenji Narahara⁴, Tohru Miyoshi-Akiyama², Teruo Kirikae⁵, Norio Ohmagari⁶

Affiliations

¹ Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku, Tokyo 162-8655, Japan; Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan.
² Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku, Tokyo 162-8655, Japan.
³ BML Inc., Kawagoe, Saitama, Japan.
⁴ Mizuho Medy Co., Ltd. R&D, Tosu, Saga, Japan.
⁵ Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama 1-21-1, Shinjuku, Tokyo 162-8655, Japan. Electronic address: tkirikae@ri.ncgm.go.jp.
⁶ Disease Control and Prevention Center, National Center for Global Health and Medicine, Tokyo, Japan.

PMID: 24910373
DOI: 10.1016/j.jiac.2014.04.014

Abstract

The spread throughout Japan of antibiotic-resistance factors in multidrug-resistant (MDR) Pseudomonas aeruginosa isolates was investigated epidemiologically, using immunochromatographic assays specific for IMP-type metallo-β-lactamases (IMPs) and aminoglycoside 6'-N-acetyltransferase [AAC(6')]-Iae and -Ib. Three hundred MDR P. aeruginosa isolates were obtained during each of two years, 2011 and 2012, from 190 hospitals in 39 prefectures in Japan. The percentage of P. aeruginosa isolates producing IMPs, AAC(6')-Iae or AAC(6')-Ib increased significantly from 170/300 (56.7%) in 2011 to 230/300 (76.7%) in 2012, with 134/170 (78.8%) in 2011 and 179/230 (77.8%) in 2012 producing both IMP and either AAC(6')-Iae or AAC(6')-Ib. The MICs of antibiotics, including cephalosporins and carbapenems, were markedly higher for isolates that did than did not produce these resistance factors. These results indicated that MDR P. aeruginosa producing IMPs, AAC(6')-Iae or AAC(6')-Ib have spread throughout Japan and that these antibiotic-resistance factors are useful markers for monitoring MDR P. aeruginosa in Japan.

Keywords: AAC; IMP; Immunochromatographic assay; Multidrug-resistance; Pseudomonas aeruginosa.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetyltransferases / metabolism*
Anti-Bacterial Agents / therapeutic use*
Bacterial Proteins / metabolism
Carbapenems / therapeutic use
Cephalosporins / therapeutic use
Drug Resistance, Multiple, Bacterial
Humans
Inosine Monophosphate / metabolism*
Japan
Microbial Sensitivity Tests
Pseudomonas Infections / drug therapy
Pseudomonas Infections / microbiology*
Pseudomonas aeruginosa / drug effects
Pseudomonas aeruginosa / enzymology*
Pseudomonas aeruginosa / isolation & purification
beta-Lactamases / biosynthesis*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Bacterial Proteins
Carbapenems
Cephalosporins
Inosine Monophosphate
Acetyltransferases
aminoglycoside N(6')-acetyltransferase
beta-Lactamases