Analysis of the released nuclear cytokine HMGB1 in human serum

Methods Mol Biol. 2014:1172:13-25. doi: 10.1007/978-1-4939-0928-5_2.

Abstract

A ubiquitous nuclear protein, the high-mobility group box 1 (HMGB1), is secreted by activated macrophages/monocytes and leaked passively from injured cells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Antibodies / chemistry
  • Blotting, Western / methods
  • Blotting, Western / standards*
  • Cell Nucleus / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • HMGB1 Protein / analysis*
  • HMGB1 Protein / metabolism
  • Humans
  • Macrophage Activation
  • Macrophages / cytology
  • Macrophages / immunology
  • Macrophages / metabolism*
  • Molecular Sequence Data
  • Peptides / chemistry
  • Peptides / immunology
  • Peroxidase / chemistry

Substances

  • Antibodies
  • HMGB1 Protein
  • HMGB1 protein, human
  • Peptides
  • Peroxidase