Abstract
A ubiquitous nuclear protein, the high-mobility group box 1 (HMGB1), is secreted by activated macrophages/monocytes and leaked passively from injured cells. HMGB1 functions as a mediator of infection- and injury-elicited inflammatory diseases. Here, we describe a semiquantitative immuno-blotting method to measure the released HMGB1 in human serum, in comparison with a commercially available HMGB1 ELISA technique.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Antibodies / chemistry
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Blotting, Western / methods
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Blotting, Western / standards*
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Cell Nucleus / metabolism
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Enzyme-Linked Immunosorbent Assay
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HMGB1 Protein / analysis*
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HMGB1 Protein / metabolism
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Humans
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Macrophage Activation
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Macrophages / cytology
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Macrophages / immunology
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Macrophages / metabolism*
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Molecular Sequence Data
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Peptides / chemistry
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Peptides / immunology
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Peroxidase / chemistry
Substances
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Antibodies
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HMGB1 Protein
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HMGB1 protein, human
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Peptides
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Peroxidase