Evaluation of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of blood isolates of Acinetobacter species

Po-Ren Hsueh; Lu-Cheng Kuo; Tsung-Chain Chang; Tai-Fen Lee; Shih-Hua Teng; Yu-Chung Chuang; Lee-Jene Teng; Wang-Huei Sheng

doi:10.1128/JCM.01233-14

Evaluation of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of blood isolates of Acinetobacter species

J Clin Microbiol. 2014 Aug;52(8):3095-100. doi: 10.1128/JCM.01233-14. Epub 2014 Jun 4.

Authors

Po-Ren Hsueh¹, Lu-Cheng Kuo², Tsung-Chain Chang³, Tai-Fen Lee⁴, Shih-Hua Teng⁵, Yu-Chung Chuang², Lee-Jene Teng⁴, Wang-Huei Sheng⁶

Affiliations

¹ Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.
² Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan.
³ Department of Medical Laboratory Science and Biotechnology, Medical College of National Cheng Kung University, Tainan, Taiwan.
⁴ Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan Department and Graduate Institute of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University, Taipei, Taiwan.
⁵ Bruker Taiwan Co., Ltd., Taipei, Taiwan.
⁶ Department of Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan whsheng@ntu.edu.tw.

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) (Bruker Biotyper) was able to accurately identify 98.6% (142/144) of Acinetobacter baumannii isolates, 72.4% (63/87) of A. nosocomialis isolates, and 97.6% (41/42) of A. pittii isolates. All Acinetobacter junii, A. ursingii, A. johnsonii, and A. radioresistens isolates (n = 28) could also be identified correctly by Bruker Biotyper.

Publication types

Evaluation Study

MeSH terms

Acinetobacter / chemistry
Acinetobacter / classification*
Acinetobacter / isolation & purification
Acinetobacter Infections / diagnosis*
Acinetobacter Infections / microbiology*
Bacteremia / diagnosis*
Bacteremia / microbiology*
Bacteriological Techniques / methods*
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*