Objective: Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.
Methodology: A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.
Results: The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.
Conclusions: The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.