An improved and versatile methodology to quantify biofilms formed on solid surfaces and exposed to the air-liquid interphase

S Paytubi; P Guirado; C Balsalobre; C Madrid

doi:10.1016/j.mimet.2014.05.020

An improved and versatile methodology to quantify biofilms formed on solid surfaces and exposed to the air-liquid interphase

J Microbiol Methods. 2014 Aug:103:77-9. doi: 10.1016/j.mimet.2014.05.020. Epub 2014 Jun 2.

Authors

S Paytubi¹, P Guirado², C Balsalobre², C Madrid²

Affiliations

¹ Departament de Microbiologia, Universitat de Barcelona, Avda. Diagonal 643, 08028 Barcelona, Spain. Electronic address: s.paytubi@gmail.com.
² Departament de Microbiologia, Universitat de Barcelona, Avda. Diagonal 643, 08028 Barcelona, Spain.

PMID: 24892512
DOI: 10.1016/j.mimet.2014.05.020

Abstract

To study pellicle formation, a new method has been developed to quantify biofilm formed on solid surfaces and exposed to air-liquid interphase. It is a versatile system since different adherent material surfaces might be tested. The methodology is a robust and reproducible approach to quantify biofilm.

Keywords: Air–liquid interphase; Biofilm; Salmonella.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Air
Bacterial Adhesion*
Biofilms*
Biomass
Surface Properties*
Time Factors