Immunodiagnosis of human and porcine cysticercosis. Detection of antibodies and parasite products

Acta Leiden. 1989;57(2):93-9.

Abstract

In this paper we report the immunodiagnosis of cysticercosis by the detection of antibodies in the cerebrospinal fluid (CSF) of patients, by standard enzyme immunoassay (EIA) which gave 90% sensitivity. We are also showing the results obtained with a double homologous polyclonal or monoclonal antibody based EIA that detects parasite products: we analysed CSF of patients and obtained sensitivities ranging from 14% to 75% for single antibody systems and 90% when analysing the samples with two or three systems simultaneously. The sera of infected pigs were also evaluated; with one monoclonal antibody we could detect 79% of positive samples. The good results obtained encourage the standardization and improvement of the technique for diagnostic as well as for epidemiologic purposes.

MeSH terms

  • Animals
  • Antibodies, Helminth / cerebrospinal fluid
  • Antigens, Helminth / isolation & purification
  • Cysticercosis / diagnosis*
  • Cysticercosis / parasitology
  • Cysticercosis / veterinary
  • Humans
  • Immunologic Tests
  • Swine
  • Swine Diseases / diagnosis
  • Swine Diseases / parasitology
  • Taenia / immunology

Substances

  • Antibodies, Helminth
  • Antigens, Helminth