Clinical signs and bacterial communities of deciduous necrotic root canals detected by PCR-DGGE analysis: research association

Viviane Andrade Cancio de Paula; Dennis de Carvalho Ferreira; Fernanda Sampaio Cavalcante; Flávia Lima do Carmo; Alexandre Soares Rosado; Laura Guimarães Primo; Kátia Regina Netto dos Santos

doi:10.1016/j.archoralbio.2014.05.012

Clinical signs and bacterial communities of deciduous necrotic root canals detected by PCR-DGGE analysis: research association

Arch Oral Biol. 2014 Aug;59(8):848-54. doi: 10.1016/j.archoralbio.2014.05.012. Epub 2014 May 13.

Authors

Viviane Andrade Cancio de Paula¹, Dennis de Carvalho Ferreira², Fernanda Sampaio Cavalcante³, Flávia Lima do Carmo³, Alexandre Soares Rosado³, Laura Guimarães Primo⁴, Kátia Regina Netto dos Santos³

Affiliations

¹ Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Disciplina de Odontopediatria da FO-UFRJ, Cidade Universitária - CCS, Caixa Postal: 68066, CEP: 21941-971 Rio de Janeiro, RJ, Brazil. Electronic address: vicancio@ig.com.br.
² Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Cidade Universitária, Av. Brigadeiro Trompowsky s/n, CEP: 21941-590 Rio de Janeiro, RJ, Brazil; Fellow by CAPES (Bolsista da CAPES) - Proc. n̊ BEX 9203 - CAPES Foundation, Ministry of Education of Brazil, Brasilia/DF 70040-020, Brazil; University of Groningen, Faculty of Mathematics and Natural Science, Microbial Ecology - Centre for Ecological and Evolutionary Studies, Nijenborgh 7, CEP: 9747AG, Groningen, Holanda, The Netherlands; Oral Medicine discipline, School of Dentistry, Graduate Program in Dentistry, Veiga de Almeida University, Rua Ibituruna, 108 - Maracanã, CEP: 20271-020 Rio de Janeiro, Brazil.
³ Department of Medical Microbiology, Institute of Microbiology, Federal University of Rio de Janeiro, Cidade Universitária, Av. Brigadeiro Trompowsky s/n, CEP: 21941-590 Rio de Janeiro, RJ, Brazil.
⁴ Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Disciplina de Odontopediatria da FO-UFRJ, Cidade Universitária - CCS, Caixa Postal: 68066, CEP: 21941-971 Rio de Janeiro, RJ, Brazil.

PMID: 24880500
DOI: 10.1016/j.archoralbio.2014.05.012

Abstract

Objective: This study sought to investigate the possible association between clinical and radiographic data of the patients with the bacterial community profiles involved in cases of necrosis in primary root canals.

Methods: Microbial community profiles for 25 samples from necrotic deciduous root canals were analyzed using the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting approach. These results were related to the clinical and radiographic data of these patients.

Results: The analysis showed a large diversity of microbial communities in necrotic deciduous root canals. The statistical results pointed out that posterior and anterior teeth were associated with <20 bands and >20 bands in PCR-DGGE method, respectively. A relationship was verified between ages >4 years old and posterior teeth and, ages ≤4 years old and anterior teeth.

Conclusions: The data showed a polymicrobial community and pointed out the association of age with necrosis in anterior and posterior teeth.

Keywords: Deciduous root canals; Denaturing gradient gel electrophoresis; Endodontic infection; Primary teeth.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques
Child
Child, Preschool
Denaturing Gradient Gel Electrophoresis
Dental Pulp Cavity / microbiology*
Female
Humans
Infant
Male
Necrosis / microbiology
Periapical Diseases / microbiology*
Polymerase Chain Reaction
Root Canal Therapy*
Tooth, Deciduous / microbiology*