miRNA modulation of SOCS1 using an influenza A virus delivery system

Leonard Izzard; Siying Ye; Kristie Jenkins; Yingju Xia; Mark Tizard; John Stambas

doi:10.1099/vir.0.063834-0

miRNA modulation of SOCS1 using an influenza A virus delivery system

J Gen Virol. 2014 Sep;95(Pt 9):1880-1885. doi: 10.1099/vir.0.063834-0. Epub 2014 May 29.

Authors

Leonard Izzard¹, Siying Ye¹, Kristie Jenkins², Yingju Xia¹, Mark Tizard², John Stambas¹

Affiliations

¹ School of Medicine, Deakin University, Geelong, Victoria, Australia.
² CSIRO, Australian Animal Health Laboratory, Geelong, Victoria, Australia.

PMID: 24876306
DOI: 10.1099/vir.0.063834-0

Abstract

Difficulties associated with efficient delivery and targeting of miRNAs to cells is hampering the real world application of miRNA technology. This study utilized an influenza A-based delivery system to express miR-155 in order to knockdown SOCS1 mRNA. Using qPCR and dual luciferase technology we show that miR-155 delivery resulted in a significant increase in cellular miR-155 which facilitated a downregulation of SOCS1 gene expression and a functional increase in IL-6 and IFN-β cytokines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Chlorocebus aethiops
Dogs
Gene Knockdown Techniques
Gene Transfer Techniques*
Genetic Vectors
Influenza A virus / genetics*
Interferon-beta / biosynthesis
Interleukin-6 / biosynthesis
Madin Darby Canine Kidney Cells
Mice
MicroRNAs / genetics*
Suppressor of Cytokine Signaling 1 Protein
Suppressor of Cytokine Signaling Proteins / biosynthesis
Suppressor of Cytokine Signaling Proteins / genetics*
Vero Cells

Substances

Interleukin-6
MicroRNAs
Mirn155 microRNA, mouse
Socs1 protein, mouse
Suppressor of Cytokine Signaling 1 Protein
Suppressor of Cytokine Signaling Proteins
Interferon-beta