Macrophage depletion prior to Neospora caninum infection results in severe neosporosis in mice

Clin Vaccine Immunol. 2014 Aug;21(8):1185-8. doi: 10.1128/CVI.00082-14. Epub 2014 May 28.

Abstract

We observed that murine macrophages showed greater activation and increased interleukin 6 (IL-6), IL-12p40, and interferon gamma (IFN-γ) production during Neospora caninum infection. Many macrophages migrated to the site of infection. Furthermore, macrophage-depleted mice exhibited increased sensitivity to N. caninum infection. This study indicates that macrophages are required for achieving protective immunity against N. caninum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Differentiation / biosynthesis
  • B7-1 Antigen / biosynthesis
  • B7-2 Antigen / biosynthesis
  • CD11b Antigen / metabolism
  • CD4-Positive T-Lymphocytes / immunology*
  • CD40 Antigens / biosynthesis
  • Cells, Cultured
  • Coccidiosis / immunology*
  • Coccidiosis / parasitology
  • Interferon-gamma / biosynthesis
  • Interleukin-12 Subunit p40 / biosynthesis
  • Interleukin-6 / biosynthesis
  • Macrophage Activation / immunology
  • Macrophages / immunology*
  • Macrophages / parasitology
  • Mice
  • Mice, Inbred C57BL
  • Neospora / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation
  • B7-1 Antigen
  • B7-2 Antigen
  • CD11b Antigen
  • CD40 Antigens
  • Cd86 protein, mouse
  • Interleukin-12 Subunit p40
  • Interleukin-6
  • monocyte-macrophage differentiation antigen
  • Interferon-gamma