Screening and monitoring zearalenone-producing Fusarium species by PCR and zearalenone by monoclonal antibodies in feed from China

Food Addit Contam Part B Surveill. 2014;7(4):282-7. doi: 10.1080/19393210.2014.925981. Epub 2014 Jun 23.

Abstract

Screening of zearalenone (ZEN)-producing species and monitoring of ZEN in feed were performed by using anti-zearalenone monoclonal antibodies. ELISA recoveries of ZEN from corn distillers dried grains with solubles (DDGS) feed, corn feed, rice bran, soybean meal, wheat bran and rapeseed dregs were between 78.6% and 88.6%. ZEN recovery from culture media was 96.3% at the spiked level of 500 µg/kg. Eighty-three samples of DDGS feed, corn feed and other fee ingredients were collected from 11 provinces of China and analysed for ZEN. Average ZEN levels were 563.4 µg/kg for DDGS feed, 393.1 µg/kg for corn feed and 65.7 µg/kg for other feed ingredients. Eighteen Fusarium species such as Fusarium graminearum which could produce ZEN were isolated from corn feed and other feed ingredients.

Keywords: China; ELISA; Fusarium; PCR; feed; zearalenone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • China
  • Food Contamination / analysis*
  • Food Microbiology*
  • Fusarium* / genetics
  • Fusarium* / metabolism
  • Humans
  • Polymerase Chain Reaction
  • Zea mays / microbiology*
  • Zearalenone / analysis*

Substances

  • Antibodies, Monoclonal
  • Zearalenone