Molecular cloning and functional analysis of a H(+)-dependent phosphate transporter gene from the ectomycorrhizal fungus Boletus edulis in southwest China

Fungal Biol. 2014 May-Jun;118(5-6):453-61. doi: 10.1016/j.funbio.2014.03.003. Epub 2014 Mar 18.

Abstract

Phosphate transporters (PTs), as entry points for phosphorus (P) in organisms, are involved in a number of P nutrition processes such as phosphate uptake, transport, and transfer. In the study, a PT gene 1632 bp long (named BePT) was cloned, identified, and functionally characterized from Boletus edulis. BePT was expected to encode a polypeptide with 543 amino acid residues. The BePT polypeptide belonged to the major facilitator superfamily and showed a high degree of sequence identity to the Pht1 family. A topology model revealed that BePT exhibited 12 transmembrane helices, divided into two halves, and connected by a large hydrophilic loop in the middle. A yeast mutant complementation analysis suggested that BePT was a functional PT which mediated orthophosphate uptake of yeast at micromolar concentrations. Green fluorescent protein-BePT fusion proteins expressed were extensively restricted to the plasma membrane in BePT transformed yeast, and its activity was dependent on electrochemical membrane potential. In vitro, quantitative PCR confirmed that the expression of BePT was significantly upregulated at lower phosphorus availability, which may enhance phosphate uptake and transport under phosphate starvation. Our results suggest that BePT plays a key role in phosphate acquisition in the ectomycorrhizal fungus B. edulis.

Keywords: Gene expression; Heterologous characterization; Phosphate uptake; Plasma membrane phosphate transporter; Yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / classification
  • Basidiomycota / genetics
  • Basidiomycota / metabolism*
  • China
  • Cloning, Molecular*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism*
  • Molecular Sequence Data
  • Mycorrhizae / classification
  • Mycorrhizae / genetics
  • Mycorrhizae / metabolism*
  • Phosphate Transport Proteins / genetics*
  • Phosphate Transport Proteins / metabolism*
  • Phosphates / metabolism
  • Phylogeny

Substances

  • Fungal Proteins
  • Phosphate Transport Proteins
  • Phosphates

Associated data

  • GENBANK/JX524166