A new stationary phase for high performance liquid chromatography: Calix[4]arene derivatized chitosan bonded silica gel

Jing Lu; Wenfen Zhang; Yanhao Zhang; Wenjie Zhao; Kai Hu; Ajuan Yu; Pu Liu; Yangjie Wu; Shusheng Zhang

doi:10.1016/j.chroma.2014.05.021

A new stationary phase for high performance liquid chromatography: Calix[4]arene derivatized chitosan bonded silica gel

J Chromatogr A. 2014 Jul 11:1350:61-7. doi: 10.1016/j.chroma.2014.05.021. Epub 2014 May 14.

Authors

Jing Lu¹, Wenfen Zhang¹, Yanhao Zhang¹, Wenjie Zhao², Kai Hu³, Ajuan Yu¹, Pu Liu⁴, Yangjie Wu¹, Shusheng Zhang⁵

Affiliations

¹ College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
² College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China; School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, PR China.
³ Henan University of Traditional Chinese Medicine, Zhengzhou 450008, PR China.
⁴ College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: liupu@zzu.edu.cn.
⁵ Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address: zhangshusheng@371.net.

PMID: 24861787
DOI: 10.1016/j.chroma.2014.05.021

Abstract

In the present paper, a new calix[4]arene derivatized chitosan bonded stationary phase (CCS4) for high performance liquid chromatography (HPLC) was synthesized and characterized. Its chromatographic performance and retention mechanism were evaluated in reversed-phase mode compared with ODS using different solute probes including mono-substituted benzenes, phenols and nucleosides. The results showed that CCS4 stationary phase could provide various interactions with solutes, such as hydrophobic, hydrophilic, π-π, and inclusion interactions. It could perform the mixed-mode separation including RP and HILIC.

Keywords: Calix[4]arene; Chitosan; HILIC; Mixed-mode separation; RPLC; Stationary phase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Calixarenes / chemistry*
Chitosan / chemistry*
Chromatography, High Pressure Liquid / instrumentation*
Hydrophobic and Hydrophilic Interactions
Organic Chemicals / chemistry
Phenols / chemistry*
Silica Gel / chemistry*

Substances

Organic Chemicals
Phenols
calix(4)arene
Calixarenes
Silica Gel
Chitosan