A luciferase-based method for assay of 5'-adenylylsulfate reductase

Anal Biochem. 2014 Sep 1:460:22-8. doi: 10.1016/j.ab.2014.05.009. Epub 2014 May 22.

Abstract

A luciferase-based method was developed for measurement of 5'-adenylylsulfate (APS) reductase (APR), an enzyme of the reductive sulfate assimilation pathway in prokaryotes and plants. APR catalyzes the two-electron reduction of APS and forms sulfite and adenosine 5'-monophospahate (AMP). The luciferase-based assay measures AMP production using an enzyme-coupled system that generates luminescence. The method is shown to provide an accurate measurement of APR kinetic properties and can be used for both endpoint and continuous assays. APR activity can be measured from pure enzyme preparations as well as from crude protein extracts of tissues. In addition, the assay is ideally suited to high-throughput sample analysis of APR activity in a microtiter dish format. The method adds new capability to the study of the biochemistry and physiology of APR.

Keywords: Adenosine 5′-monophosphate; Adenosine 5′-phosphosulfate; Luciferase; Reductase; Sulfate assimilation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Monophosphate / biosynthesis
  • Animals
  • Enzyme Assays / methods*
  • Luciferases / chemistry
  • Luciferases / metabolism*
  • Luminescent Measurements
  • Oxidoreductases Acting on Sulfur Group Donors / metabolism*
  • Ulva / enzymology
  • Zea mays / enzymology

Substances

  • Adenosine Monophosphate
  • Luciferases
  • Oxidoreductases Acting on Sulfur Group Donors
  • adenylylsulfate reductase