Randomly arrayed G-quadruplexes for label-free and real-time assay of enzyme activity

Chem Commun (Camb). 2014 Jul 4;50(52):6875-8. doi: 10.1039/c4cc03103b.

Abstract

Randomly arrayed G-quadruplexes can serve as an efficient peroxidase-mimic DNAzyme and provide a novel and facile method to detect terminal deoxynucleotidyl transferase (TdT). Moreover, this G-rich sequence binding to the thioflavin T (ThT) dye can be applied in real-time fluorescent detection of TdT activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles
  • Biological Assay*
  • Biosensing Techniques
  • DNA Nucleotidylexotransferase / analysis*
  • DNA, Catalytic / chemistry
  • DNA, Catalytic / metabolism*
  • G-Quadruplexes*
  • Hemin / chemistry
  • Hemin / metabolism*
  • Peroxidase / metabolism*
  • Spectrometry, Fluorescence
  • Thiazoles / chemistry
  • Thiazoles / metabolism*

Substances

  • Benzothiazoles
  • DNA, Catalytic
  • Thiazoles
  • thioflavin T
  • Hemin
  • Peroxidase
  • DNA Nucleotidylexotransferase