Abstract
Randomly arrayed G-quadruplexes can serve as an efficient peroxidase-mimic DNAzyme and provide a novel and facile method to detect terminal deoxynucleotidyl transferase (TdT). Moreover, this G-rich sequence binding to the thioflavin T (ThT) dye can be applied in real-time fluorescent detection of TdT activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzothiazoles
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Biological Assay*
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Biosensing Techniques
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DNA Nucleotidylexotransferase / analysis*
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DNA, Catalytic / chemistry
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DNA, Catalytic / metabolism*
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G-Quadruplexes*
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Hemin / chemistry
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Hemin / metabolism*
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Peroxidase / metabolism*
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Spectrometry, Fluorescence
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Thiazoles / chemistry
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Thiazoles / metabolism*
Substances
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Benzothiazoles
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DNA, Catalytic
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Thiazoles
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thioflavin T
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Hemin
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Peroxidase
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DNA Nucleotidylexotransferase