Myotube formation on gelatin nanofibers - multi-walled carbon nanotubes hybrid scaffolds

Serge Ostrovidov; Xuetao Shi; Ling Zhang; Xiaobin Liang; Sang Bok Kim; Toshinori Fujie; Murugan Ramalingam; Mingwei Chen; Ken Nakajima; Faten Al-Hazmi; Hojae Bae; Adnan Memic; Ali Khademhosseini

doi:10.1016/j.biomaterials.2014.04.021

Myotube formation on gelatin nanofibers - multi-walled carbon nanotubes hybrid scaffolds

Biomaterials. 2014 Aug;35(24):6268-77. doi: 10.1016/j.biomaterials.2014.04.021. Epub 2014 May 13.

Authors

Affiliations

¹ WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan.
² Department of Eco-Machinery System, Korea Institute of Machinery and Materials, Daejeon 305-343, South Korea.
³ Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Tokyo 162-8480, Japan.
⁴ WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan; Centre for Stem Cell Research, A Unit of the Institute for Stem Cell Biology and Regenerative Medicine, Christian Medical College Campus, Vellore 632002, India.
⁵ Department of Physics, King Abdulaziz University, Jeddah 21569, Saudi Arabia.
⁶ College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea.
⁷ Center of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
⁸ WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan; Department of Physics, King Abdulaziz University, Jeddah 21569, Saudi Arabia; Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA; Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: alik@rics.bwh.harvard.edu.

PMID: 24831971
DOI: 10.1016/j.biomaterials.2014.04.021

Abstract

Engineering functional muscle tissue requires the formation of densely packed, aligned, and mature myotubes. To enhance the formation of aligned myotubes with improved contractibility, we fabricated aligned electrospun gelatin multi-walled carbon nanotubes (MWNTs) hybrid fibers that were used as scaffolds for the growth of myoblasts (C2C12). The MWNTs significantly enhanced myotube formation by improving the mechanical properties of the resulting fibers and upregulated the activation of mechanotransduction related genes. In addition, the fibers enhanced the maturation of the myotubes and the amplitude of the myotube contractions under electrical stimulation (ES). Such hybrid material scaffolds may be useful to direct skeletal muscle cellular organization, improve cellular functionality and tissue formation.

Keywords: C2C12; Carbon nanotubes; Electrospinning; Gelatin fibers; Myotube.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / drug effects
Cell Differentiation / genetics
Cell Line
Cell Survival / drug effects
Cell Survival / genetics
Cross-Linking Reagents / pharmacology
Electric Stimulation
Gelatin / pharmacology*
Gene Expression Regulation / drug effects
Mice
Microscopy, Fluorescence
Muscle Fibers, Skeletal / cytology*
Muscle Fibers, Skeletal / drug effects
Nanofibers / chemistry*
Nanofibers / ultrastructure
Nanotubes, Carbon / chemistry*
Nanotubes, Carbon / ultrastructure
Sus scrofa
Tissue Scaffolds / chemistry*

Substances

Cross-Linking Reagents
Nanotubes, Carbon
Gelatin