Applying antibiotic selection markers for nematode genetics

Eric Cornes; Cécile A L Quéré; Rosina Giordano-Santini; Denis Dupuy

doi:10.1016/j.ymeth.2014.04.016

Applying antibiotic selection markers for nematode genetics

Methods. 2014 Aug 1;68(3):403-8. doi: 10.1016/j.ymeth.2014.04.016. Epub 2014 May 10.

Authors

Eric Cornes¹, Cécile A L Quéré², Rosina Giordano-Santini³, Denis Dupuy⁴

Affiliations

¹ Cancer and Human Molecular Genetics, Bellvitge Biomedical Research Institute-IDIBELL, Hospitalet de Llobregat, Barcelona 08908, Spain; Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France.
² Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France.
³ Molecular and Cellular Neurobiology Laboratory, The University of Queensland, Queensland Brain Institute, Qld 4072, Australia.
⁴ Univ. Bordeaux, IECB, Laboratoire ARNA, F-33600 Pessac, France; INSERM, U869, Laboratoire ARNA, F-33000 Bordeaux, France. Electronic address: d.dupuy@iecb.u-bordeaux.fr.

PMID: 24821108
DOI: 10.1016/j.ymeth.2014.04.016

Abstract

Antibiotic selection markers have been recently developed in the multicellular model organism Caenorhabditis elegans and other related nematode species, opening great opportunities in the field of nematode transgenesis. Here we describe how these antibiotic selection systems can be easily combined with many well-established genetic approaches to study gene function, improving time- and cost-effectiveness of the nematode genetic toolbox.

Keywords: Antibiotic; C. elegans; Transformation marker; Transgenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Biomarkers
Caenorhabditis elegans / drug effects
Caenorhabditis elegans / genetics*
Gene Transfer Techniques*
Mutation
Selection, Genetic*
Transformation, Genetic*

Substances

Anti-Bacterial Agents
Biomarkers