Isolation and gut microbiota modulation of antibiotic-resistant probiotics from human feces

Diagn Microbiol Infect Dis. 2014 Aug;79(4):405-12. doi: 10.1016/j.diagmicrobio.2014.04.002. Epub 2014 Apr 13.

Abstract

Antibiotic-resistant probiotics may be advantageous for antibiotic-induced gut microbiota imbalance. In this article, we aimed to isolate antibiotic-resistant bacteria as potential probiotics. Feces from 3 healthy adults and 2 infants were used to isolate the antibiotic-resistant bacteria. Then we established gut microbiota imbalance mice model by antibiotics treatment and used it to assess the effect of the probiotics. Finally, we identified 8 isolates, and 6 of them were used as probiotics cocktail. Number of anaerobe, lactobacilli, and Bifidobacterium in feces were higher in the probiotic group (9.47±0.35 log10CFU/g, 8.74±0.18 log10CFU/g, 7.24±0.38 log10CFU/g, respectively) compared with model group (P<0.05). Richness and diversity index of probiotic group (19.79±0.29 and 2.95±0.06, respectively) were larger than model group (P<0.05). Diarrhea and mucosal edema had been alleviated during probiotic treatment. Our results validated that bacteriotherapy was available to treat gut microbiota imbalance.

Keywords: Bacteriotherapy; Diarrhea; Gut microbiota imbalance; Probiotics cocktail.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Bacteria / classification
  • Bacteria / drug effects
  • Bacteria / genetics
  • Conjugation, Genetic
  • Disk Diffusion Antimicrobial Tests
  • Drug Resistance, Bacterial* / genetics
  • Feces / microbiology*
  • Gastrointestinal Tract / microbiology*
  • Humans
  • Infant
  • Infant, Newborn
  • Metagenome
  • Mice
  • Microbial Sensitivity Tests
  • Microbiota*
  • Probiotics*
  • RNA, Bacterial
  • RNA, Ribosomal, 16S

Substances

  • Anti-Bacterial Agents
  • RNA, Bacterial
  • RNA, Ribosomal, 16S