Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays

Sophie de Bentzmann; Christophe Bordi

doi:10.1007/978-1-4939-0473-0_19

Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays

Methods Mol Biol. 2014:1149:225-41. doi: 10.1007/978-1-4939-0473-0_19.

Authors

Sophie de Bentzmann¹, Christophe Bordi

Affiliation

¹ Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), UMR7255 CNRS-Aix Marseille Université, Institut de Microbiologie de la Méditerranée, Marseille cédex 20, France.

PMID: 24818909
DOI: 10.1007/978-1-4939-0473-0_19

Abstract

In Pseudomonas aeruginosa, identification of new partners of a protein of interest could give precious clues to decipher a biological process in which this protein is involved. However, genes encoding for partners of a protein of interest are unknown and frequently scattered throughout the genome. We describe herein the construction and the use of pan-genomic bacterial two-hybrid libraries to identify new partners of a protein of interest encoded by P. aeruginosa.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclic AMP / biosynthesis
Genes, Reporter
Genome, Bacterial
High-Throughput Screening Assays / methods*
Plasmids / metabolism
Protein Binding
Pseudomonas aeruginosa / genetics
Pseudomonas aeruginosa / metabolism*
Reproducibility of Results
Two-Hybrid System Techniques*

Substances

Cyclic AMP