The rational design of a highly sensitive and selective fluorogenic probe for detecting nitric oxide

Chem Commun (Camb). 2014 Jun 21;50(49):6475-8. doi: 10.1039/c4cc01142b. Epub 2014 May 12.

Abstract

A rationally designed small-molecule fluorogenic probe for nitric oxide (NO) detection based on a new switching mechanism has been developed. Attaching a NO-responsive dihydropyridine pendant group to a fluorophore led to a probe that displays a very high sensitivity to NO concentrations down to the low nM range and a very high specificity to NO while being insensitive to other oxidative oxygen/nitrogen species that often interfere with the sensing of NO.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Coumarins / chemical synthesis
  • Coumarins / chemistry*
  • Dihydropyridines / chemistry
  • Electron Transport
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Hydrogen-Ion Concentration
  • Mice
  • Microscopy, Fluorescence*
  • Niacin / analogs & derivatives*
  • Niacin / chemical synthesis
  • Niacin / chemistry
  • Nitric Oxide / analysis*
  • Reactive Nitrogen Species / chemistry
  • Reactive Oxygen Species / chemistry
  • Spectrometry, Fluorescence*

Substances

  • Coumarins
  • Dihydropyridines
  • Fluorescent Dyes
  • Reactive Nitrogen Species
  • Reactive Oxygen Species
  • Niacin
  • Nitric Oxide
  • 1,4-dihydropyridine