Tetrodotoxin-producing Bacillus sp. from the ribbon worm (Nemertea) Cephalothrix simula (Iwata, 1952)

Timur Yu Magarlamov; Irina A Beleneva; Alexey V Chernyshev; Andrey D Kuhlevsky

doi:10.1016/j.toxicon.2014.04.015

Tetrodotoxin-producing Bacillus sp. from the ribbon worm (Nemertea) Cephalothrix simula (Iwata, 1952)

Toxicon. 2014 Jul:85:46-51. doi: 10.1016/j.toxicon.2014.04.015. Epub 2014 May 9.

Authors

Timur Yu Magarlamov¹, Irina A Beleneva², Alexey V Chernyshev³, Andrey D Kuhlevsky²

Affiliations

¹ A.V. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok, Russia; School of Biomedicine, Far Eastern Federal University, Vladivostok, Russia. Electronic address: biotimur@yandex.ru.
² A.V. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok, Russia.
³ A.V. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok, Russia; School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia.

PMID: 24813332
DOI: 10.1016/j.toxicon.2014.04.015

Abstract

Specimens of the toxic ribbon worm Cephalothrix simula from the Sea of Japan were screened for tetrodotoxin-producing bacteria. A single TTX-producing bacterial strain (No 1839) was isolated from tissues of C. simula and studied by immunohistochemical methods (including immunoelectron and immunofluorescent microscopies) with anti-TTX antibodies. Sequencing of 16S rRNA gene of the strain 1839 showed that it is most likely Bacillus sp. CU040510-015 and Bacillus asahii. Based on its morphological and biochemical properties, however we suppose that the isolated Bacillus sp. 1839 should be classified as representing a new species. Microdistribution of TTX in bacterial cell was investigated under electron microscope by immunoenzymatic methods. TTX was concentrated in the forespore and free spores, but it was not detected in the vegetative cells of Bacillus sp. 1839. We suggest that release of free mature spores from sporangium of Bacillus sp. 1839 leads to appearance of toxin in tissues of C. simula. Confocal laser-scanning microscopy (CLSM) method with anti-TTX antibodies can be recommended for preliminary detection of apparent TTX accumulation.

Keywords: Bacillus; Forespore; Immunogold; Nemertea; Tetrodotoxin; Tetrodotoxin-producing bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacillus / classification
Bacillus / isolation & purification
Bacillus / physiology*
Bacillus / ultrastructure
Immunohistochemistry
Invertebrates / microbiology*
Japan
Microscopy, Confocal
Microscopy, Electron, Transmission
Microscopy, Immunoelectron
Molecular Typing
Neurotoxins / biosynthesis*
Pacific Ocean
Protein Transport
Spores, Bacterial / metabolism
Spores, Bacterial / ultrastructure
Tetrodotoxin / biosynthesis*

Substances

Neurotoxins
Tetrodotoxin