An evidence in vitro for the influence of bisphenol A on uterine leiomyoma

Eur J Obstet Gynecol Reprod Biol. 2014 Jul:178:80-3. doi: 10.1016/j.ejogrb.2014.03.052. Epub 2014 Apr 13.

Abstract

Objective: To study the effect and mechanism of environmental estrogens bisphenol A (BPA) on uterine leiomyoma (UL) cells.

Methods: Primary cultures and subcultures of human UL cells, which were identified by immunocytochemical staining with a monoclonal anti-α-smooth muscle actin antibody, were performed. The viability of cells in different concentration of bisphenol A of 24h, 48h and 72h were analyzed by CCK-8. The expressions of mRNA of ERα, IGF-1 and VEGF in all groups were detected by real-time quantitative PCR assay, and then the expressions of proteins detected by Western blot assay.

Results: BPA promoted the growth of UL cells and the expressions of ERα, IGF-1 and VEGF, which had positive correlation with the concentration and action time of BPA treatment.

Conclusion: BPA promotes the growth of leiomyoma cells. The expressions of IGF-1, VEGF can be up-regulated by ERα, which may be possible mechanism of BPA promote the growth of leiomyoma cells.

Keywords: Bisphenol A; ERα; Environmental estrogen; IGF-1; Uterine leiomyoma; VEGF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzhydryl Compounds / pharmacology*
  • Cell Proliferation / drug effects
  • Estrogen Receptor alpha / biosynthesis
  • Estrogens / pharmacology*
  • Female
  • Humans
  • Insulin-Like Growth Factor I / biosynthesis
  • Leiomyoma / metabolism*
  • Phenols / pharmacology*
  • Tumor Cells, Cultured
  • Uterine Neoplasms / metabolism*
  • Vascular Endothelial Growth Factor A / biosynthesis

Substances

  • Benzhydryl Compounds
  • ESR1 protein, human
  • Estrogen Receptor alpha
  • Estrogens
  • Phenols
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • Insulin-Like Growth Factor I
  • bisphenol A