Identification of sex-linked DNA markers by AFLP in Portunus trituberculatus

Gene. 2014 Jul 10;544(2):216-9. doi: 10.1016/j.gene.2014.04.034. Epub 2014 Apr 21.

Abstract

To investigate the mechanism of sex determination of crabs, amplified fragment-length polymorphism DNA analysis was applied to identify sex-specific markers for Portunus trituberculatus. Eleven pairs of primers were used to amplify DNA isolated from male and female crabs. A total of 481 and 499 fragments were amplified for females and males, respectively, and the ratios of polymorphic fragments were 20.59-65.52% and 27.27-62.22%, respectively. Distribution difference values between female and male groups for 10 polymorphisms were greater than 40%; Cluster analysis revealed that Nei's genetic distances for 57 polymorphisms clustered according to sex. The results indicate that a difference at the DNA level exists between female and male crabs and provide data for further study on sex determination.

Keywords: AFLP; Cluster analysis; Portunus trituberculatus; Sex-linked.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amplified Fragment Length Polymorphism Analysis
  • Animals
  • Brachyura / genetics*
  • DNA / genetics
  • DNA Primers / genetics
  • Female
  • Genetic Markers
  • Male
  • Nucleic Acid Amplification Techniques
  • Polymorphism, Single Nucleotide
  • Sex Determination Processes / genetics*

Substances

  • DNA Primers
  • Genetic Markers
  • DNA