Differentiation, polarization, and migration of human induced pluripotent stem cell-derived neural progenitor cells co-cultured with a human glial cell line with radial glial-like characteristics

Biochem Biophys Res Commun. 2014 May 16;447(4):683-8. doi: 10.1016/j.bbrc.2014.04.070. Epub 2014 Apr 19.

Abstract

Here we established a unique human glial cell line, GDC90, derived from a human glioma and demonstrated its utility as a glial scaffold for the polarization and differentiation of human induced pluripotent stem cell-derived neural progenitor cells (iPSC-NPCs). When co-cultured with GDC90 cells, iPSC-NPCs underwent rapid polarization and neurite extension along the radially spreading processes of the GDC90 cells, and showed migratory behavior. This method is potentially useful for detailed examination of neurites or for controlling neurites behavior for regenerative medicine.

Keywords: Glioma; Induced pluripotent stem cells; Neuronal migration; PiggyBac transposon; Radial glia; Regenerative medicine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / physiology
  • Cell Line
  • Cell Line, Tumor
  • Cell Movement / physiology
  • Cell Polarity / physiology
  • Cell Shape
  • Coculture Techniques / methods
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / physiology*
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Membranes, Artificial
  • Nerve Regeneration
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / physiology*
  • Neurites / physiology
  • Neuroglia / cytology*
  • Neuroglia / physiology*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Regenerative Medicine / methods
  • Transfection

Substances

  • Luminescent Proteins
  • Membranes, Artificial
  • Recombinant Proteins