The distribution of MspI-induced breaks in human lymphocyte chromosomes and its relationship to common fragile sites

B Porfirio; B Tedeschi; P Vernole; D Caporossi; B Nicoletti

doi:10.1016/0027-5107(89)90142-5

The distribution of MspI-induced breaks in human lymphocyte chromosomes and its relationship to common fragile sites

Mutat Res. 1989 Aug;213(2):117-24. doi: 10.1016/0027-5107(89)90142-5.

Authors

B Porfirio¹, B Tedeschi, P Vernole, D Caporossi, B Nicoletti

Affiliation

¹ Department of Public Health and Cell Biology, 2nd University of Rome, Italy.

PMID: 2474762
DOI: 10.1016/0027-5107(89)90142-5

Abstract

The restriction endonuclease MspI (cleavage site C/CGG) induces chromosomal breaks in human lymphocytes. The breakpoints are distributed non-randomly along the chromosomes and the pattern of MspI-induced breakage depends on the recovery time (20 h or 6 h). Chromosomal bands preferentially involved in breakage are likely to coincide with bands where common fragile sites are located.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Aberrations*
Chromosome Banding
Chromosome Fragile Sites
Chromosome Fragility*
Deoxyribonuclease HpaII
Deoxyribonucleases, Type II Site-Specific* / metabolism
Humans
Karyotyping
Lymphocytes / ultrastructure*

Substances

Deoxyribonuclease HpaII
Deoxyribonucleases, Type II Site-Specific