Long-term pre-exposure of pheochromocytoma PC12 cells to endocrine-disrupting chemicals influences neuronal differentiation

Neurosci Lett. 2014 Jun 6:570:1-4. doi: 10.1016/j.neulet.2014.04.014. Epub 2014 Apr 16.

Abstract

This study examined the effects of exposure to low concentrations (0.1-100 nM) of bisphenol A (BPA) or nonylphenol (NP) on neuronal differentiation in pheochromocytoma PC12 cells. Pre-exposure to BPA for a week or a month, but not for a day, decreased neuronal differentiation in PC12 cells. Likewise, one week's pre-exposure to NP also inhibited neuronal differentiation in these cells. The inhibitory effects were still observed when PC12 cells were treated with BPA or NP for a week, followed by a week's withdrawal. These findings suggest that long-term exposure of PC12 cells to low concentrations of BPA or NP leads to changes in the cellular machinery responsible for neuronal differentiation, and these changes might be retained within PC12 cells.

Keywords: Bisphenol A; Neuronal differentiation; Nonylphenol; PC12 cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzhydryl Compounds / toxicity
  • Cell Death / drug effects
  • Cell Differentiation
  • Endocrine Disruptors / toxicity*
  • Estrogen Receptor alpha / genetics
  • Estrogen Receptor alpha / metabolism
  • Estrogen Receptor beta / genetics
  • Estrogen Receptor beta / metabolism
  • Neurons / cytology
  • Neurons / drug effects*
  • Neurons / metabolism
  • Oxidative Stress / drug effects
  • PC12 Cells
  • Phenols / toxicity
  • Pheochromocytoma
  • Rats
  • Receptors, Estrogen / genetics
  • Receptors, Estrogen / metabolism
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism

Substances

  • Benzhydryl Compounds
  • Endocrine Disruptors
  • Esrrg protein, rat
  • Estrogen Receptor alpha
  • Estrogen Receptor beta
  • Gper1 protein, rat
  • Phenols
  • Receptors, Estrogen
  • Receptors, G-Protein-Coupled
  • nonylphenol
  • bisphenol A