Differential effects of chemoattractants on mast cell recruitment in vivo

Cell Immunol. 2014 May-Jun;289(1-2):86-90. doi: 10.1016/j.cellimm.2014.03.013. Epub 2014 Apr 3.

Abstract

Rats were injected with rat recombinant (rr) IL3, rrSCF, rrIL-3+rrSCF, rrRANTES and LTB4. Six hours after subcutaneous injection of rrIL-3 or rrIL-3+rrSCF, there was an increase in mast cell numbers in the skin and spleen. Peritoneal mast cells were recruited following i.p. injection of rrIL-3, but with rrIL-3+rrSCF recruitment was delayed. Immunostaining with a mast cell specific antibody showed that immature orthochromatic mast cells were being recruited. rrIL-3 induced recruitment of mast cells to the peritoneal cavity was blocked by anti-integrin antibodies. Mast cell recruitment depended on the target tissue and the time of exposure to the chemoattractant.

Keywords: IL-3; In vivo; LTB4; Mast cells; RANTES; Recruitment; SCF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Movement / immunology
  • Chemokine CCL5 / pharmacology
  • Chemotactic Factors / immunology*
  • Chemotactic Factors / pharmacology
  • Interleukin-3 / pharmacology
  • Leukotriene B4 / pharmacology
  • Male
  • Mast Cells / drug effects
  • Mast Cells / immunology*
  • Peritoneal Cavity
  • Rats
  • Rats, Wistar
  • Recombinant Proteins / pharmacology
  • Skin / cytology
  • Skin / immunology
  • Spleen / cytology
  • Spleen / immunology
  • Stem Cell Factor / pharmacology

Substances

  • Chemokine CCL5
  • Chemotactic Factors
  • Interleukin-3
  • Recombinant Proteins
  • Stem Cell Factor
  • Leukotriene B4