Stimulation of osteogenic and angiogenic ability of cells on polymers by pulsed laser deposition of uniform akermanite-glass nanolayer

Chengtie Wu; Dong Zhai; Hongshi Ma; Xiaomin Li; Yali Zhang; Yinghong Zhou; Yongxiang Luo; Yueyue Wang; Yin Xiao; Jiang Chang

doi:10.1016/j.actbio.2014.03.035

Stimulation of osteogenic and angiogenic ability of cells on polymers by pulsed laser deposition of uniform akermanite-glass nanolayer

Acta Biomater. 2014 Jul;10(7):3295-306. doi: 10.1016/j.actbio.2014.03.035. Epub 2014 Apr 12.

Authors

Chengtie Wu¹, Dong Zhai², Hongshi Ma², Xiaomin Li², Yali Zhang², Yinghong Zhou³, Yongxiang Luo², Yueyue Wang², Yin Xiao³, Jiang Chang⁴

Affiliations

¹ State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: chengtiewu@mail.sic.ac.cn.
² State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
³ Institute of Health & Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland 4059, Australia.
⁴ State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: jchang@mail.sic.ac.cn.

PMID: 24726444
DOI: 10.1016/j.actbio.2014.03.035

Abstract

Polymer biomaterials have been widely used for bone replacement/regeneration because of their unique mechanical properties and workability. Their inherent low bioactivity makes them lack osseointegration with host bone tissue. For this reason, bioactive inorganic particles have been always incorporated into the matrix of polymers to improve their bioactivity. However, mixing inorganic particles with polymers always results in inhomogeneity of particle distribution in polymer matrix with limited bioactivity. This study sets out to apply the pulsed laser deposition (PLD) technique to prepare uniform akermanite (Ca2MgSi2O7, AKT) glass nanocoatings on the surface of two polymers (non-degradable polysulfone (PSU) and degradable polylactic acid (PDLLA)) in order to improve their surface osteogenic and angiogenic activity. The results show that a uniform nanolayer composed of amorphous AKT particles (∼30 nm) of thickness 130 nm forms on the surface of both PSU and PDLLA films with the PLD technique. The prepared AKT-PSU and AKT-PDLLA films significantly improved the surface roughness, hydrophilicity, hardness and apatite mineralization, compared with pure PSU and PDLLA, respectively. The prepared AKT nanocoatings distinctively enhance the alkaline phosphate (ALP) activity and bone-related gene expression (ALP, OCN, OPN and Col I) of bone-forming cells on both PSU and PDLLA films. Furthermore, AKT nanocoatings on two polymers improve the attachment, proliferation, VEGF secretion and expression of proangiogenic factors and their receptors of human umbilical vein endothelial cells (HUVEC). The results suggest that PLD-prepared bioceramic nanocoatings are very useful for enhancing the physicochemical, osteogenic and angiogenic properties of both degradable and non-degradable polymers for application in bone replacement/regeneration.

Keywords: Angiogenic; Bone regeneration; Osteogenic; Polymers; Pulsed laser deposition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Animals
Bone and Bones / metabolism
Ceramics*
Gene Expression Profiling
Glass*
Lasers
Mice
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Nanostructures*
Neovascularization, Physiologic*
Osteogenesis*
Polymers / chemistry*

Substances

Polymers
Glass ceramics