Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction

F S Campos; A C Franco; M T Oliveira; R Firpo; G Strelczuk; F E Fontoura; M I R Kulmann; S Maidana; S A Romera; F R Spilki; A D Silva; S O Hübner; P M Roehe

doi:10.1016/j.vetmic.2014.03.012

Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction

Vet Microbiol. 2014 Jun 25;171(1-2):182-8. doi: 10.1016/j.vetmic.2014.03.012. Epub 2014 Mar 25.

Authors

F S Campos¹, A C Franco², M T Oliveira², R Firpo², G Strelczuk², F E Fontoura², M I R Kulmann², S Maidana³, S A Romera³, F R Spilki⁴, A D Silva⁵, S O Hübner⁶, P M Roehe⁷

Affiliations

¹ Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil. Electronic address: camposvet@gmail.com.
² Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil.
³ Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), N. Repetto y Los Reseros S/N, CC25 (B1712WAA), Castelar, Buenos Aires, Argentina.
⁴ Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale University, Rodovia RS-239 2755, Novo Hamburgo, CEP 93.352-000, Rio Grande do Sul (RS), Brazil.
⁵ Embrapa CNPSA, BR 153, Km 110, Post Box 21, Concórdia, CEP 89.700-000, Santa Catarina (SC), Brazil.
⁶ Laboratory of Virology, Department of Preventive Veterinary Medicine, Faculty of Veterinary Medicine, Federal University of Pelotas (UFPel), University Campus, Post Box 354, Pelotas, CEP 96.010-900, Rio Grande do Sul (RS), Brazil.
⁷ Laboratory of Virology, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90.050-170, Rio Grande do Sul (RS), Brazil; Institute for Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92.990-000, Rio Grande do Sul (RS), Brazil.

PMID: 24725448
DOI: 10.1016/j.vetmic.2014.03.012

Abstract

Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.

Keywords: Bovine herpesvirus; Internal control; Latency; Ovine herpesvirus; PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cattle Diseases / diagnosis*
Cattle Diseases / virology
Cell Line
Coinfection
DNA, Viral / genetics
Herpesviridae Infections / diagnosis
Herpesviridae Infections / veterinary*
Herpesviridae Infections / virology
Herpesvirus 1, Bovine / physiology
Herpesvirus 2, Bovine / genetics
Herpesvirus 4, Bovine / genetics
Herpesvirus 5, Bovine / physiology
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction / veterinary*
Trigeminal Ganglion / virology*

Substances

DNA, Viral

Associated data

GENBANK/JQ958306
GENBANK/JQ958307