Abstract
A new class of polymer capsules with an in-built endocytic pH-coupled fluorescence switch is reported. These capsules display reversible "on/off" fluorescence in response to cellular pH variations. Using this system, the high-throughput quantification between surface-bound and internalized capsules is demonstrated. This system allows a fundamental study of the interaction between nanoengineered materials and biological systems at a cellular level.
Keywords:
cell uptake; internalization; layer-by-layer; nanotechnology; polymer capsules; sensors.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bone Marrow Cells / chemistry
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Bone Marrow Cells / metabolism
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Capsules / administration & dosage
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Capsules / chemistry
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Capsules / pharmacokinetics
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Cell Line
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Dendritic Cells / chemistry
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Dendritic Cells / metabolism
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Drug Carriers / administration & dosage*
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Drug Carriers / chemistry
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Drug Carriers / pharmacokinetics
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Drug Delivery Systems / instrumentation
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Drug Delivery Systems / methods*
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Endocytosis / physiology*
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Fluorescent Dyes / administration & dosage*
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacokinetics
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Hydrogen-Ion Concentration
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Mice
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Microscopy, Fluorescence
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Models, Molecular
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Polymethacrylic Acids / administration & dosage*
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Polymethacrylic Acids / chemistry
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Polymethacrylic Acids / pharmacokinetics
Substances
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Capsules
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Drug Carriers
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Fluorescent Dyes
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Polymethacrylic Acids
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poly(2-(diisopropylamino)ethyl methacrylate)