Based on the remote images in 2001 and 2010, the source and sink areas of urban heat island (UHI) in Shenyang City, Northeast China were determined by GIS technique. The effect of urban regional landscape pattern on UHI effect was assessed with land surface temperature (LST), area rate index (CI) of the source and sink areas and intensity index (LI) of heat island. The results indicated that the land use type changed significantly from 2001 to 2010, which significantly changed the source and sink areas of UHI, especially in the second and third circle regions. The source and sink areas were 94.3% and 5.7% in the first circle region, 64.0% and 36.0% in the third circle region in 2001, while they were 93.4% and 6.6%, 70.2% and 29.8% in 2010, respectively. It suggested that the land use pattern extended by a round shape in Shenyang led to the corresponding UHI pattern. The LST in the study area tended to decrease from the first circle region to the third. The UHI intensity was characterized with a single center in 2001 and with several centers in 2010, and the grade of UHI intensity was in a decreasing trend from 2001 to 2010. The absolute value of CI increased from the first circle region to the third, and the L1 was close to 1, suggesting the change in land use pattern had no significant influence on UHI in Shenyang.