Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells

Narisorn Kongruttanachok; Yvon E Cayre; Hans Knecht; Sabine Mai

doi:10.1532/LH96.12023

Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells

Lab Hematol. 2014 Mar 1;20(1):2-6. doi: 10.1532/LH96.12023.

Authors

Narisorn Kongruttanachok¹, Yvon E Cayre², Hans Knecht³, Sabine Mai⁴

Affiliations

¹ Manitoba Institute of Cell Biology, Cancer Care Manitoba, The Genomic Centre for Cancer Research and Diagnosis, The University of Manitoba, Winnipeg, Manitoba, Canada Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
² Hôpital Robert Debré and Pierre, Marie Curie University, Paris, France.
³ Division of Haematology/Oncology, CHUS University Hospital, Sherbrooke, Quebec, Canada.
⁴ Manitoba Institute of Cell Biology, Cancer Care Manitoba, The Genomic Centre for Cancer Research and Diagnosis, The University of Manitoba, Winnipeg, Manitoba, Canada.

PMID: 24694783
DOI: 10.1532/LH96.12023

Abstract

We describe a method to isolate small mononucleated Hodgkin (H) cells from multinucleated Reed Sternberg (RS) cells of Hodgkin lymphoma using the ScreenCell filter device. This filtration-based approach lends itself to future clinical applications in that it enables the separation of H and RS cells from lymph node biopsies, bone marrow aspirates, pleural effusions, and blood, including the isolation of monoclonal Hodgkin precursor cells from the blood.

Keywords: Hodgkin; Reed Sternberg; cell separation; filtration device.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Separation / methods
Hodgkin Disease / pathology*
Humans
Reed-Sternberg Cells / pathology*
Sentinel Lymph Node Biopsy