Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing

Olivier Clermont; Julia K Christenson; Anne-Sophie Daubié; David M Gordon; Erick Denamur

doi:10.1016/j.mimet.2014.03.008

Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing

J Microbiol Methods. 2014 Jun:101:24-7. doi: 10.1016/j.mimet.2014.03.008. Epub 2014 Mar 28.

Authors

Olivier Clermont¹, Julia K Christenson², Anne-Sophie Daubié³, David M Gordon², Erick Denamur⁴

Affiliations

¹ INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
² Division of Evolution, Ecology & Genetics, Research School of Biology, The Australian National University, Canberra ACT 0200, Australia.
³ INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Nord, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France.
⁴ INSERM, IAME, UMR 1137, F-75018 Paris, France; Univ Paris Diderot, IAME, UMR 1137, Sorbonne Paris Cité, F-75018 Paris, France. Electronic address: erick.denamur@inserm.fr.

PMID: 24685601
DOI: 10.1016/j.mimet.2014.03.008

Abstract

We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.

Keywords: Allele-specific PCR; B2 phylogroup; B2 sub-group; Escherichia coli; Sequence type complex.

MeSH terms

Alleles
Animals
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Escherichia coli / genetics*
Escherichia coli Infections / microbiology
Genes, Bacterial / genetics*
Humans
Multilocus Sequence Typing / methods*
Polymerase Chain Reaction / methods*

Substances

DNA, Bacterial