Two novel clerodane diterpenenes with NGF-potentiating activities from the twigs of Croton yanhuii

Fitoterapia. 2014 Jun:95:229-33. doi: 10.1016/j.fitote.2014.03.012. Epub 2014 Mar 29.

Abstract

Nerve growth factor (NGF) and analog reagents to promote the neurite outgrowth of nerve cells against the neuron degeneration are expected to be potentially useful for the medical treatment of Alzheimer's disease. In our focus on the discovery of bioactive diterpenes, we investigated the chemical constituents of the plant Croton yanhuii. This investigation led to the isolation and identification of two novel clerodane diterpenes (1 and 2). Their structures were elucidated on the basis of extensive 1D and 2D NMR (COSY, HMQC, HMBC, and NOESY) and mass (ESIMS and HR-ESIMS) spectroscopic data analyses. Further biological screenings showed that both of the compounds enhanced NGF-mediated neurite outgrowth from PC12 cells.

Keywords: Alzheimer's disease; Clerodane diterpene; Croton yanhuii; Nerve growth factor (NGF)-potentiating activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alzheimer Disease / drug therapy*
  • Animals
  • Croton / chemistry*
  • Diterpenes, Clerodane / chemistry
  • Diterpenes, Clerodane / isolation & purification*
  • Diterpenes, Clerodane / pharmacology
  • Diterpenes, Clerodane / therapeutic use
  • Drug Synergism
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Nerve Growth Factor / metabolism
  • Neurites / drug effects*
  • PC12 Cells
  • Plants, Medicinal
  • Rats

Substances

  • Diterpenes, Clerodane
  • crotonpene A
  • crotonpene B
  • Nerve Growth Factor